RESEARCH TECHNICIAN II
Duke University

This Position is Partially Grant Funded

School of Medicine:

Established in 1930, Duke University School of Medicine is the youngest of the nation's top medical schools. Ranked tenth among its peers, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where traditional barriers are low, interdisciplinary collaboration is embraced, and great ideas accelerate translation of fundamental scientific discoveries to improve humanhealth locally and around the globe.

Comprised of 2,400 faculty physicians and researchers, the Duke University School of Medicine along with the Duke University School of Nursing and Duke University Health System create Duke Health. Duke Health is a world-class health care network. Founded in 1998 to provide efficient, responsive care, the health system offers a full network of health services and encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Private Diagnostic Clinic, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.

RESEARCH TECHNICIAN II

The Duke Human Vaccine Institute (DHVI), an international leader in the fight against major infectious diseases, is seeking a highly motivated individual for the position of Research Technician II. Investigators at the DHVI conduct basic and translational research to develop novel vaccines, therapeutics and diagnostics.

The Duke Regional Biocontainment Laboratory (RBL) is a division of the DHVI and supports a wide portfolio of research programs. The RBL has three collaborative core units: Immunology, Virology, and Microbiology. The Research Technician will work with the RBL Immunology team to prepare specimens for PBMC isolation, quantitative PCR, flow cytometry, ELISA, Luminex and other immunological assays. In addition, the technician will collect and interpret data and perform general laboratory maintenance and inventory management.

The ideal candidate will have a Bachelor's degree in biological science or a related field. Previous laboratory experience in immunology, microbiology, virology and/or molecular biology is highly preferred. Experience with quantitative PCR, molecular biology, flow cytometery and/or ELISAs preferred but not required.

RESPONSIBILITIES:

• Prepare specimens for and perform immunological procedures, including but not limited to: PBMC isolation, quantitative PCR, flow cytometry, ELISA and Luminex assays.
• General laboratory maintenance including but not limited to: laboratory organization, preparation of solutions, waste processing, ordering/preparation/stocking of consumables and reagents, routine equipment calibration, maintenance of accurate sample and equipment inventories.
Coordinate equipment maintenance performed by outside vendors.
• Attend all required training.
• Confer with Immunology Unit Manager / Principal Investigator to review work assignments and develop plans for experiments; make minor modifications to existing laboratory procedures and techniques to meet the needs of particular studies as required.
• Manage and complete all assigned tasks/projects in accordance with standardized operating procedures and in full compliance with all safety protocols.

• Complete and maintain written and electronic documentation of research activities in real time according to standardized record keeping procedures.

• Collect and organize raw data into reports for interpretation and analysis.
• Perform additional duties as requested by Immunology Unit Manager and RBL/DHVI leadership.

REQUIREMENTS:

Must be detail-oriented, well organized with strong communication skills and able to work in a team environment.

The successful applicant must be eligible and willing to:

• Complete necessary training and certification for independent work at Biological Safety Level 3 (BSL3).
• Work with infectious biological agents (viruses and bacteria) handled at BSL2 and BSL3.
• Comply with vaccination requirements.
• Complete Tier 1 Select Agent registration.

Minimum Qualifications

Education

Work generally requires a bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.

Experience

None required above education/training requirement. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE

Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.

Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas-an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.

Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essentialjob functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.

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